Combinatorial effects of metformin and glucose on the immune evasion of breast cancer 4T1 cells
- Laboratory of Stem Cell Research and Application, University of Science, Ho Chi Minh City, Viet Nam
- Viet Nam National University Ho Chi Minh City, Ho Chi Minh City, Viet Nam
- iet Nam National University Ho Chi Minh City, Ho Chi Minh City, Viet Nam
- Stem Cell Institute, University of Science, Ho Chi Minh City, Viet Nam
- Laboratory of Cancer Research, University of Science, Ho Chi Minh City, Viet Nam
Abstract
Introduction: Metformin is one current medicine used to treat type 2 diabetes. Numerous studies have shown that high metformin concentrations have an anti-cancer effect. Therefore, this study aimed to examine whether combining various metformin and glucose concentrations affects mouse breast cancer cell proliferation, migration, and expression of immune escape-related genes.
Methods: This study assessed 12 glucose and metformin combinations: four glucose concentrations (0, 0.5, 1.0, and 4.5 g/L) and three metformin concentrations (0, 2.0, and 5.0 mM). Mouse breast cancer 4T1 cells were cultured in RPMI 1640 media containing these 12 combinations at 37?C with 5% CO2. The combinatorial effects of metformin and glucose were evaluated based on 4T1 cell proliferation, migration, and expression of immune escape-related genes.
Results: Combining 2 mM metformin with 4.5 g/L glucose concentration inhibited 4T1 cell proliferation, migration, and expression of immune escape-related genes.
Conclusion: Our findings provide more information about the anticancer effects of metformin under high glucose conditions, help explain why metformin effectively treats cancer in patients with type 2 diabetes, and suggest combining metformin with glucose in anticancer treatment.