http://bmrat.org/index.php/BMRAT <p>&nbsp;</p> <p>Biomedical Research and Therapy - Vietnamese Journal for Medical Biotechnology and Medicine Incorporating Advances in Regenerative Medicine publishes 12 peer-reviewed issues each year, covering a wide range of biomedical and clinical sciences. Unlike many open-access journals, which charge authors for publication while providing free reader access, Biomedical Research and Therapy does not require fees for subscription, submission, processing, publication, or color image reproduction. Recognized internationally, this journal is committed to disseminating high-quality research in an open-access format, emphasizing basic, translational, and clinical studies on molecular therapeutics and cellular therapies. It includes research involving animal models and clinical trials. The rigorous peer-review process ensures that only scientifically, technically, and ethically sound articles adhering to standard reporting guidelines are published. The journal’s editorial policies are in strict alignment with standards set by the International Committee of Medical Journal Editors (ICMJE), the World Association of Medical Editors (WAME), and the Committee on Publication Ethics (COPE), upholding the highest principles of publication ethics.</p> Biomedpress en-US Biomedical Research and Therapy 2198-4093 <p>Copyright The Author(s) 2017. This article is published with open access by <a href="http://www.biomedpress.org/" target="_blank">BioMedPress</a>. This article is distributed under the terms of the&nbsp;<a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">Creative Commons Attribution License (CC-BY 4.0)</a> which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.&nbsp;</p> http://bmrat.org/index.php/BMRAT/article/view/933 <p><strong>Introduction</strong>: Cellular senescence is an extensively researched issue aimed at influencing the aging process. A novel research direction involves studying the potential of plant extracts on this process. <em>Boesenbergia pandurata</em> (Roxb.) Schltr, also known as <em>Boesenbergia rotunda</em> (L.) Mansf, is a herb with significant potential for research into its effects on aging. Furthermore, it is crucial to study cellular senescence models to accurately assess the impact of various agents on the aging process.</p> <p><strong>Methods</strong>: In this study, the ethanol extract of <em>Boesenbergia pandurata</em> (Roxb.) Schltr was evaluated for its potential effects on certain characteristics of senescent fibroblasts derived from foreskin, which were induced by etoposide. The cells were treated with 20 μM etoposide for 48 hours to induce senescence.</p> <p><strong>Results</strong>: The study demonstrated that treating fibroblasts with 20 μM etoposide for 48 hours induces senescence characteristics. Additionally, administering the ethanol extract of Boesenbergia pandurata at a concentration of 15 μg/ml ameliorated several features of senescence in fibroblasts. The treatment resulted in a 27% reduction in cell size (p &lt; 0.05), a 1.2-fold decrease in SA-β-Galactosidase enzyme activity (p &lt; 0.0001), and reduced gene expression of <em>p16, p21</em>, and <em>p53</em>.</p> <p><strong>Conclusion</strong>: We established a senescent fibroblast model using 20 μM etoposide and demonstrated that the finger root extract at 15 μg/ml improved various senescence-related cellular characteristics, suggesting its potential as an anti-aging agent.</p> Phuc Hong Vo Nghia Minh Do Sinh Truong Nguyen ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2024-11-30 2024-11-30 11 11 6869 6882 10.15419/bmrat.v11i11.933 title description none g http://bmrat.org/index.php/BMRAT/article/view/934 <p><strong>Objective</strong>: Inflammation plays a pivotal role in the pathogenesis of stroke. However, the proteins that initiate inflammatory responses remain unclear. In this study, we utilize proteomics to identify the core protein in acute ischemic stroke (AIS) patients and verify the relationship of the protein with NOD-like receptor protein 3 (NLRP3).</p> <p><strong>Methods</strong>: Peripheral blood from AIS patients and patients without AIS was collected and analyzed using a quantitative proteomic method (label-free) to screen for differential proteins. Subsequently, the differential protein was validated by ELISA. Additionally, a middle cerebral artery occlusion (MCAO) model was utilized to explore the relationship between mannose-binding lectin 2 (MBL2) and NLRP3. Co-immunoprecipitation (Co-IP) and western blot were also used to validate the interaction between the proteins and NLRP3.</p> <p><strong>Results</strong>: A total of ten AIS patients and controls were enrolled in the proteomics study. Compared with the control group, a total of 49 proteins were identified as potential proteins. Among these proteins, MBL2 was notably increased in the AIS group and selected for further analysis. Subsequent ELISA analysis confirmed a significant elevation of MBL2 levels in stroke patients (P &lt; 0.001). Further, in animal studies, Co-IP assays showed an interaction between MBL2 and NLRP3 proteins in cerebral tissue after ischemic infarction. Western blot results demonstrated that the expression levels of NLRP3 and MBL2 were significantly increased in MCAO rats.</p> <p><strong>Conclusions</strong>: Our results suggest that MBL2 may be one of the promoters of inflammation by interacting with NLRP3 in AIS patients.</p> Bin Zhu Xinchen Li Yue Tian Hua Yang Mingfen Wu Shaoqing Wang Bin Wang Zhigang Zhao ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2024-11-30 2024-11-30 11 11 6883 6890 10.15419/bmrat.v11i11.934 title description none g